Methylene Blue
Methylene Blue
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Product Information:
Quick Specifications
- Bottle Content: 2 FL OZ (60 mL)
- Concentration: 1% Methylene Blue Solution
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Each 1 mL Contains:
- 10 mg Methylene Blue (USP Grade)
- 50 mg Magnesium (as L-Threonate)
- Purity: USP Grade
- Key Features: ALCOHOL-FREE, FORMALDEHYDE-FREE
Storage: Store at room temperature (15°C—25°C). Keep container tightly closed in a dry, well-ventilated place. Protect from direct sunlight and moisture.
What Is Methylene Blue?
Methylene Blue, first synthesized by German chemist Heinrich Caro in 1876, is one of the most historically significant compounds in medicine and biology. Originally developed as a synthetic blue dye for the textile industry, it quickly found its place in science. It was identified by Nobel laureate Paul Ehrlich for its selective staining properties of nerve cells and microbes, leading to its distinction as the "first magic bullet" in pharmacology.
Its remarkable history includes being the first fully synthetic drug, the first antiseptic used in clinical practice, and a foundational treatment for malaria for over 60 years. Today, Methylene Blue is the subject of intensive modern research for its unique properties, particularly its role as a redox-cycling agent and its effects on cellular energy metabolism.
Methylene Blue Structure & Chemical Information
Methylene Blue is a heterocyclic aromatic compound belonging to the phenothiazine class. Its distinct deep blue color in its oxidized state is due to its chromophore system. As a salt, it is readily soluble in water.
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IUPAC Name: 3,7-bis(dimethylamino)phenothiazin-5-ium chloride
- Chemical Formula: C₁₆H₁₈ClN₃S
- Molar Mass: 319.85 g/mol
Methylene Blue Research
Methylene Blue's wide range of biological activities is a subject of ongoing scientific investigation. Its primary mechanism of action is related to its ability to cycle between its oxidized (blue) and reduced (colorless) forms.
Mitochondrial Enhancement & Bioenergetics
At low concentrations, Methylene Blue acts as a unique "electron cycler" within the mitochondria. Research indicates it can accept electrons from NADH and shuttle them directly to Cytochrome C in the electron transport chain (ETC), effectively bypassing Complexes I and II. This process helps maintain mitochondrial membrane potential and supports the continued production of ATP (the primary cellular energy currency), particularly under conditions of metabolic stress or mitochondrial dysfunction. By enhancing the efficiency of the ETC, Methylene Blue may improve cellular respiration and energy output.
Neuroprotective Properties
Methylene Blue has demonstrated significant neuroprotective potential in various preclinical research models. Its proposed mechanisms include:
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Antioxidant Effects: It can scavenge free radicals and reduce oxidative stress within neuronal cells.
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Inhibition of Tau Aggregation: Studies suggest that Methylene Blue can prevent the aggregation of tau proteins into neurofibrillary tangles, a primary pathological hallmark of Alzheimer's disease.
- Mitochondrial Support: By boosting mitochondrial function in neurons, it may help protect them from age-related decline and neurotoxic insults.
Monoamine Oxidase (MAO) Inhibition
Methylene Blue is a potent, reversible inhibitor of Monoamine Oxidase A (MAO-A), an enzyme responsible for breaking down key neurotransmitters like serotonin, melatonin, norepinephrine, and dopamine. Due to this action, Methylene Blue has been studied for its effects on mood and cognition. However, this MAO-A inhibition is also the reason for a critical safety consideration: co-administration with serotonergic agents (such as SSRIs) can lead to a dangerous excess of serotonin, known as serotonin syndrome. This property is a key focus in pharmacological research and safety profiling.
For Research Purposes Only This product is for laboratory research only by qualified scientists. Not for human use, medical treatment, or veterinary applications. Follow all safety protocols.


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